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KMID : 1149220170250040527
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Korean Journal of Oriental Medical Prescription
2017 Volume.25 No. 4 p.527 ~ p.536
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Effect of Acer tegmentosum Maxim. extract on differentiation of osteoblastic Primary calvarial osteoblasts cells
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Oh Tae-Woo
Shim Ki-Shuk
Kim Kwang-Youn
Cho Won-Kyung
Park Kwang-Il
Ma Jin-Yeul
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Abstract
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Objectives : The present study, to confirm the osteoblast differentiation effects of Acer tegmentosum Maxim. (AT) extract.
Methods : In this experiment, cell viability, Alizarin red S assay, and Alkaline phosphatase (ALP) activity with AT extract (50, 100 ㎍/㎖). Also, we studied the expression of differentiation regulator with AT extract in primary calvarial osteoblasts cells (pOB).
Results : As a result of AT treatment, we determined that AT extract stimulates ALP activity and alizarin red activities in the pOB cells for mineralization for 18 days. Moreover, these factors increasing osteogenic markers such as Runt-related transcription factor2 (Runx2), osteocalcin (OC), osteopontin, osterix, smad1, smad5, activating transcription factor4 (ATF4) and collagen type Ⅰ alpha 1.
Conclusions : These results indicate that AT extract have effect on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of bone diseases.
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KEYWORD
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Osteoblast , Differentiation , Acer tegmentosum Maxim , primary calvarial osteoblasts cells , osteogenic marker
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